2017 Fiscal Year Final Research Report
Search for AhR endogenous ligands by metabolomics
| Project/Area Number |
26460377
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Keywords | CYP1A / P450 / 多環芳香族炭化水素 / ダイオキシン / AhR / メダカ / 変異体 / メタボローム |
|---|
| Outline of Final Research Achievements |
Medaka deficient for CYP1A, a highly conserved phase I detoxifying enzyme, was created by TILLING method. This CYP1A 'knockout' medaka reacted to lower (about one tenth) concentrations of polycyclic aromatic hydrocarbons than their wild type littermates. Interestingly, AhR signaling pathway is dramatically activated in unexposed CYP1A knockouts compared to the wild type, suggesting that there are endogenous ligands for AhR that are metabolized by CYP1A in vivo. The metabolome analysis revealed that CYP1A is possibly involved in nucleic acid metabolism.
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| Free Research Field |
環境医学
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