2016 Fiscal Year Final Research Report
in vivo analysis of Mesenchymal stem cells using Runx2 conditional KO mouse
| Project/Area Number |
26460387
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Pathological medical chemistry
|
|---|
| Research Institution | Okayama University (2016)
Kanazawa University (2014-2015) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
Hinoi Eiichi 金沢大学, 薬学系, 准教授 (70360865)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 間葉系幹細胞 / Runx2 |
|---|
| Outline of Final Research Achievements |
The cellular origin and essential period for Runx2 function during osteoblast differentiation in intramembranous ossification remain poorly understood. Paired related homeobox 1 (Prx1) is expressed in craniofacial mesenchyme, and Runx2 deficiency in Prx1+-derived cells (Runx2prx1－/－ mice) resulted in defective intramembranous ossification. Double-positive cells for Prx1-GFP and stem cell antigen-1 (Sca1) (Prx1+Sca1+ cells) in the calvaria expressed Runx2 at lower levels and were more homogeneous and primitive as compared with Prx1+Sca1－ cells. These findings indicate that the essential period of Runx2 function on intramembranous ossification would begin at the Prx1+Sca1+ mesenchymal stem cell stage and end at the Osx+Prx1－Sca1－ osteoblast precursor stage.
|
| Free Research Field |
幹細胞医学
|
