Study on a novel cholix toxin produced by Vibrio cholerae

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460533
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka Prefecture University
• Principal Investigator: YAMASAKI SHINJI 大阪府立大学, 生命環境科学研究科, 教授 (70221653)
• Co-Investigator(Renkei-kenkyūsha): Asakura Masahiro 大阪府立大学, 生命環境科学研究科, 客員研究員 (10529142)
• Research Collaborator: Awasthi Sharda P. 大阪府立大学, 生命環境科学研究科, 客員研究員 (30751888)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: コリックストキシン / コレラ菌 / ADP-リボシルトランスフェラーゼ

Outline of Final Research Achievements

The PCR-RFLP assay, which can detect and differentiate three distinct cholix toxin (chxA) genes in Vibrio cholerae, was established. By using this PCR-RFLP assay, a novel chxA gene termed as chxAIV gene was detected. Furthermore, bead-ELISA was constructed to quantify ChxAI to ChxAIII and the production level of ChxAI, ChxAII and ChxIII were determined. The production level of ChxA varied from strain to strains. High level and low level of ChxA-producing V. cholerae strains were intraperitoneally injected to mice. The mice lethality was higher in high level of ChxA-producer than low level of ChxA-producer. Taken together, our results indicate that there are at least 4 chxA variant-genes in v. cholerae and ChxA may be an important virulence factor for mice lethality, indicating that ChxA could be involved in extraintestinal infection.

Free Research Field

細菌学