Development of rapid and simple diagnostic assays for 10 food poisoning bacteria and viruses using monoclonal antibodies

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460807
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: University of Hyogo
• Principal Investigator: Kitamoto Noritoshi 兵庫県立大学, 環境人間学部, 教授 (70145928)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 単クローン抗体 / 感染型食中毒 / 毒素型食中毒 / 下痢症ウイルス / 迅速簡便診断法 / イムノクロマト法

Outline of Final Research Achievements

Monoclonal antibodies (MAbs) are powerful tools for the study of microorganism. A panel of MAbs against food poisoning bacteria and viruses would be valuable for the development of rapid, simple and specific diagnostic assays such as Enzyme-linked immunosorbent assay (ELISA) and immunochromatography (IC) applied for multiple samples. We obtained 8 MAbs to each bacterium, enterotoxigenic Escherichia coli, Salmonella enteritidis, Vibrio parahaemolyticus, Campylobacter jejuni, Verotoxin producing Escherichia coli, Staphylococcus aureus, Clostridium perfringens and Bacillus cereus. We confirmed these MAbs were specific to each bacterium by an ELISA test and IC. The IC is and will make it possible to screen for and detect food poisoning in most of laboratories or institutes.

Free Research Field

微生物学、感染症学、食品衛生学