Analysis of characteristics of cellular subfractions in adipose tissue-derived stromal cells for development of liver repair/regenerative therapy

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460995
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kanazawa University
• Principal Investigator: Sakai Yoshio 金沢大学, 医学系, 准教授 (80401925)
• Research Collaborator: SEKI Akihiro 金沢大学, 附属病院 ; YAMATO Masatoshi 金沢大学, 医薬保健学総合研究科恒常性制御学, 大学院生 ; NASTI Alessandro 金沢大学, 医薬保健学総合研究科恒常性制御学, 大学院生 ; YOSHIDA Keiko 金沢大学, 技術補佐員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 肝臓学 / 肝再生 / 脂肪組織由来間質細胞

Outline of Final Research Achievements

In this study, we analyzed the cellular fractions of murine uncultured adipose tissue derived stromal cells and assessed their characteristics. In addition, we investigated their effect on the liver disease condition using murine liver disease models. We found that the 10-20% of uncultured adipose tissue-derived stromal cells expressed CD45 antigen. CD45+ cells were disclosed to be characteristics of M2 phenotype macrophage in view of surface antigen analysis as well as gene expression analysis. Administration of the entire ulcutred adipose tissue derived stromal cells and their CD45+ fraction were effective to acute murine hepatitis. Thus, these results indicated that the relative importance of CD45+ cellular fraction of the uncultured adipose tissue derived stromal cells in the context of their immunomodulatory effect.

Free Research Field

医歯薬学