Analysis of neuronal death control target 14-3-3 protein / HMGB1 / Beclin1

2017 Fiscal Year Final Research Report

• Project/Area Number: 26461282
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Tokyo Medical University
• Principal Investigator: Umahara Takahiko 東京医科大学, 医学部, 准教授 (70266477)
• Co-Investigator(Kenkyū-buntansha): 秋元 治朗 東京医科大学, 医学部, 教授 (10212440) ; 羽生 春夫 東京医科大学, 医学部, 主任教授 (10228520) ; 清水 聰一郎 東京医科大学, 医学部, 講師 (10385031) ; 宮澤 啓介 東京医科大学, 医学部, 主任教授 (50209897) ; 橋本 孝朗 東京医科大学, 医学部, 講師 (60266517) ; 赫 寛雄 東京医科大学, 医学部, 准教授 (70307338) ; 織田 順 東京医科大学, 医学部, 主任教授 (60459500)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 14-3-3蛋白 / HMGB1 / Beclin1 / 神経細胞死

Outline of Final Research Achievements

14-3-3 eta isoform was located in the anterior horn cells of spinal cord of amyotrophic lateral sclerosis. Co-localization with phosphorylated TDP43 was also confirmed. We pointed out the involvement of 14-3-3 protein eta isoform in the process involving phosphorylated TDP - 43 from the nucleus to the cytoplasm and involved in anterior horn cell death. We confirmed the cytoplasmic localization of HMGB1 in neurons around the ischemic core in the acute phase of cerebral infarction. It can contribute as a translational Research of a novel cerebral infarction therapy. Blood HMGB1 concentrations in blood of 29 control subjects, 84 cases of Alzheimer's disease, 8 cases of Parkinson's disease and 25 cases of dementia of Lewy bodies were measured by ELISA method( 5.4, 6.6, 10.7, 8.1 ng / ml). We confirmed localization of autophagy - related protein Beclin 1 in carotid arteriosclerotic lesions and pointed out the involvement of autophagy in arteriosclerosis.

Free Research Field

老年神経科学