Biomarker study for multiple sclerosis with Imaging Mass Spectrometry

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Doshisha University
• Principal Investigator: Ikegawa Masaya 同志社大学, 生命医科学部, 教授 (60381943)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 多発性硬化症 / バイオマーカー / イメージング質量分析法 / プロテオミクス / 多変量解析

Outline of Final Research Achievements

Experimental autoimmune encephalomyelitis (EAE) is the most commonly used experimental model for the human inflammatory demyelinating disease, multiple sclerosis (MS). In this study, we applied matrix-assisted laser desorption/ionization (MALDI) mass imaging mass spectrometry (IMS) for EAE mice brain and spinal cord.Here we systematically visualized the distribution of proteins and peptides from brains and spinal cords of the mice during EAE development. Using multivariate analysis, area specific proteomic pattern was extracted in wild type mice and aberrant distribution was noticed in EAE mice even from early to onset stage of the diseases. For the clarification of pathogenesis of MS, we consider proteomic IMS on brain and spinal cord sections from EAE mice is a valuable approach.

Free Research Field

ゲノム医科学