2016 Fiscal Year Final Research Report
Genome wide methylation analysis for homeostasis of pancreatic beta-cells
| Project/Area Number |
26461351
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | DNAメチル化 / 糖尿病 / 遺伝子 / 発現制御 / 細胞・組織 / 膵β細胞 / 内分泌 / 転写因子 |
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| Outline of Final Research Achievements |
Relationship between cell function, gene expression and DNA methylation was analyzed in pancreatic beta-cells. In the terminal differentiation process for maturation of pancreatic islets, genome-wide inhibition in gene expression was observed, in which DNA methylation was involved. These genes were downregulated in FACS-sorted mature beta-cells but upregulated in the islets of diabetes model mice. Demethylation of promoter of Mafb, one of these genes downregulated in mature beta-cells, was observed with increased promoter activity and gene expression in glucotoxic beta-cell model. These results suggested that DNA methylation is involved in gene expression and function of beta-cells in the process of terminal differentiation and deterioration in diabetes.
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| Free Research Field |
医歯薬学
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