Discovery of Dwarfism Mouse Model by Deletion of PPAR gamma1 Specific Promoter.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461367
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Saitama Medical University
• Principal Investigator: INOUE Ikuo 埼玉医科大学, 医学部, 教授 (60232526)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: PPARγ1 / PPARγ2 / プロモータ / スプライシングバリアント / 脂肪細胞分化誘導 / ノックアウト(KO)ホモマウス

Outline of Final Research Achievements

We discovered a new splicing valiant (SV) transcribed from exon C of PPARγ1, that is previously reported promoter different from exon B of PPARγ1 (Fig.1A)(Takahashi S, Inoue I, et al. J Atheroscler Thromb 17:73-83, 2010). It proved that a new splicing valiant (SV) is involved in induction of adipocyte differentiation as well as PPARγ2(Y Takenaka, I Inoue: PLoS One 8: e 65583, 2013). We established this site specific knockout (KO) homo mice (Fig.1A) and was found to be embryonic lethal (Fig.1C). On the other hand, conventionally known exon A1 specific KO homo mice were growing normally until 18.5th fertilization (Fig.2).
Currently, exon C specific adipocyte-specific conditional gene KO mouse has also been prepared.

Free Research Field

脂質代謝