Development of a novel hematopoietic stem cell transplantation using T-cell receptor gene transduction into hematopoietic stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461450
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Ehime University
• Principal Investigator: Taichi Azuma 愛媛大学, 医学部附属病院, 講師 (10396252)
• Co-Investigator(Renkei-kenkyūsha): Yasukawa Masaki 愛媛大学, 大学院医学系研究科, 教授 (60127917)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 白血病 / がん免疫療法 / T細胞レセプター / 遺伝子治療

Outline of Final Research Achievements

We performed the series of experiment in order to establish the novel hematopoietic stem cell transplantation independent on graft-versus-host disease (GVHD) using transduction of HLA-A24-restricted WT1-specific T-cell receptor (TCR) gene into hematopoietic stem cells. Human hematopoietic stem cells were transduced with HLA-A24-restricted and WT1-specific TCR gene and they were transplanted in HLA-A24-transgenic mice. WT1-specific and HLA-A24-restricted human cytotoxic T lymphocytes (CTL) were detected in humanized mice. These CTL exerted HLA-A24-restricted cytotoxicity against human leukemia cells. Our study shows the feasibility of a novel hematopoietic stem cell transplantation using transduction of cancer-specific TCR gene into hematopoietic stem cells.

Free Research Field

血液学、腫瘍免疫学、細胞治療