2016 Fiscal Year Final Research Report
Screening of natural medicines with chemokine receptor antagonists for allergy therapy
| Project/Area Number |
26461495
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Kindai University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ケモカイン / ケモカイン受容体 / 漢方薬 |
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| Outline of Final Research Achievements |
Chemokine receptors CCR3 and CCR4 have been paid attention as potent therapeutic targets for allergic diseases. First, we screened 80 crude drugs/herbs to identify candidate substances using chemotaxis assay. Ephedra Herb inhibited the chemotaxis mediated by both CCR3 and CCR4. Thus, we examined inhibitory effects of ephedrine, a major component of Ephedra Herb. However, ephedrine exhibited little effects on the chemotaxis mediated by CCR3, CCR4, and CCR8. Therefore, we fractionated Ephedra Herb into four subfractions and examined the inhibitory effects of each subfraction. Ethyl acetate-insoluble fraction exhibited the inhibitory effects on chemotaxis and calcium mobilization mediated by CCR3 and CCR4 most significantly. Taken together, our data suggest that these crude drugs/herbs might be useful sources to develop new drugs targeting Th2-mediated allergic diseases.
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| Free Research Field |
免疫学
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