Dynamics of cellular components of bullae in bullous pemphigoid

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461664
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Osaka City University
• Principal Investigator: Tsuruta Daisuke 大阪市立大学, 大学院医学研究科, 教授 (90382043)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: focal adhesion / BP-IgG / 水疱性類天疱瘡

Outline of Final Research Achievements

We analyzed the compensation of focal adhesion (FA) adhesion after disruption of hemidesmosomes (HD) by bullous pemphigoid (BP-IgG) onto keratinocyte cell culture. The results suggested that expression of FA was inversely up-regulated after down-regulation of HD by BP-IgG, chronologically. The size of FA was increased and the dynamics of FA was decreased after BP-IgG treatment onto keratinocyte by several experiments. This phenomenon maybe explained by compensatory up-regulation of "strength of attachment" by FA from weakend "strength of attachment" by HD. On the other hand, we cannot directly see "strength of attachment" by FAs in itself. We consider that the "strength of attachment" by FA is too weak to be detected by conventional analytical method by us. We have a plan to see it after development of new equipments in the future.

Free Research Field

皮膚科学