2016 Fiscal Year Final Research Report
Functional analysis of suicide-related genes in brain
| Project/Area Number |
26461718
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Watanabe Masahiko 北海道大学, 医学研究科, 教授 (70210945)
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| Research Collaborator |
Kusumi Ichiro 北海道大学, 医学研究科, 教授 (30250426)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 自殺 / GWAS / 動物モデル / 神経幹細胞 |
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| Outline of Final Research Achievements |
The genetic research of complete suicide is behind other mental problems because it is extremely difficult to obtain tissue samples of completed suicide. Under the difficult situation, we performed a GWAS using 500 samples of suicide completers, and found two candidates of suicide-related gene; PTCHD3 and RAB18.
Here, we investigated the function of these candidate genes in chronic stressed rats and mouse neural stem cells. Stressed rats showed higher PTCHD3 expression in prefrontal cortex and hippocampus, and lower RAB18 expression in amygdala. In mouse neural stem cells with RAB18 knockdown, cell proliferation and survival were significantly increased compared to controls. Our results suggest aberrant expression of these suicide-related genes may be associated with brain abnormalities in stress conditons. Replication GWAS of suicide completers and further in vivo and in vitro analyses are needed in future studies.
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| Free Research Field |
分子精神医学
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