Investigation of stability of calcified plaque by miRNA profiling

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Nagoya City University
• Principal Investigator: KATANO Hiroyuki 名古屋市立大学, 大学院医学研究科, 准教授 (30295612)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: carotid plaque / calcification / microRNA / microarray / PCR

Outline of Final Research Achievements

Ten plaques removed from carotid endarterectomy patients were classified into high- and low-calcified plaques on the basis of Agatston calcium score. Microarray analysis for miRNA profiles was performed followed by validation with miRNA quantitative real-time (qRT) PCR analysis. Genes which satisfied total gene signal (TGS) >50 and [Log2 ratio <-1 or >1 were selected. Among carefully selected 70 angiogenesis or calcification-related transcripts, reliably expressed miRNAs marking median and high context scores with miRSearch and FDR <0.1 by the two-group comparison were also extracted .In high-calcified carotid plaques, specific profile for miRNA may be shown and expression of hsa-miR-4530 and hsa-miR-133b had inverse correlation with calcium score in plaques, suggesting miRNA may play a modulating role in calcification in plaque and plaque stability.

Free Research Field

脳神経外科