2016 Fiscal Year Final Research Report
Regeneration using adipose-derived stem cells and fluorescent imaging evaluation for a defect after bone and soft tissue tumor surgery
| Project/Area Number |
26462271
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 脂肪由来幹細胞 |
|---|
| Outline of Final Research Achievements |
We compared osteogenic and adipogenic differentiation potency between MSCs isolated and cultured from normal adipose tissues and atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) from the same patients. We found greater osteogenic differentiation potency in ALT/WDL-derived MSCs than in normal fat-derived MSCs. In adipogenic differentiation, contrary to our expectations, more fatty acid droplets were observed in normal fatderived MSCs than in ALT/WDL-derived MSCs. In osteogenic differentiation, as expected, MSCs derived from ALT/WDL had higher differentiation potency than normal fat-derived MSCs. The gap of osteogenic differentiation potency based on ALP staining between ALT/WDL-derived MSCs and normal fat-derived MSCs disappeared as Nutlin-3 concentration increased, suggesting that greater osteogenic differentiation could be caused by amplied MDM2 in ALT/WDL.
|
| Free Research Field |
再生医療
|
