2016 Fiscal Year Final Research Report
Effect of D-amino acid oxidase on spinal synaptic transmission
| Project/Area Number |
26462346
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | Ｄ-アミノ酸オキシダーゼ / NK1 internalization / ホルマリン試験 / ブレインビジョン |
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| Outline of Final Research Achievements |
To clarify the influence of nociception on spinal synaptic transmission, we assessed the NK1 recepter internalization (NK1Ri) and optical synaptic imaging using brain vision. The nocicetive behavior induced by formalin injection was significantly enhanced in mutant mice lacking DAO. The number of NK1Ri positive neurons was increased by formalin injection in ipsilateral dorsal horn. Furthermore, electrical stimulation of dorsal root ganglion attenuated the fluorescent change of voltage-sensitive dye in dorsal horn.
These results suggest that DAO is involved to the nociceptive synaptic transmission in spinal dorsal horn.
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| Free Research Field |
痛みが脊髄シナプス伝達に与える影響
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