2016 Fiscal Year Final Research Report
Prevention of induction of quinolone-resistance by inhibition of drug efflux pump in uropathogenic E. coli
| Project/Area Number |
26462455
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 尿路病原性大腸菌 / キノロン耐性 / セフェム耐性 / 薬剤排出ポンプ / シークエンス |
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| Outline of Final Research Achievements |
We induced developed fluoroquinolone-resistant strains by the exposure to fluoroquinolone, followed by the final development of high-resistant isolates. In the developed fluoroquinolone-resistant isolates, additional mutations were found in gyrA, parC and parE. Further, quantitative analysis of mRNA expression of efflux pumps and their regulators expression revealed remarkable increase of marA expression, which is a positive regulator of efflux pump.
Another interesting finding was that these developed fluoroquinolone-resistant E. coli strains showed resistance against several cephalosporin as 8 to 32 times higher MIC than the original strains. We have started the whole-genome sequence of these strains to find the mutation which are different from their original strains.
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| Free Research Field |
尿路感染症
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