Fundamental medical research of the retinal regeneration using tissue stem / progenitor cells, immortalized cells, induced pluripotent stem cells derived from iris tissue.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462701
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Fujita Health University
• Principal Investigator: Naoki Yamamoto 藤田保健衛生大学, 共同利用研究推進施設, 准教授 (00267957)
• Research Collaborator: IMAIZUMI Kazuyosi ; ISOGAI Sumito ; HIRAMATSU Noriko ; YAMASHITA Hiromi ; YAMADA Harumoto ; TAJIMA Kaori
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: iPS細胞 / 網膜再生 / 不死化細胞 / 組織幹／前駆細胞 / 再生医療 / 電気生理 / 虹彩

Outline of Final Research Achievements

We discovered that the iris tissue stem / progenitor cells could culture for a long term by the coating agent and the repressor using culture methods of the induced pluripotent stem (iPS) cells.
The immortalization cells and the iPS cells derived from iris were able to differentiate for the expression of specific for nerve and retina cell marker by using the two phases-style new differentiation method. These differentiated cells had the physiologic neurotransmission nerve cell function. In addition, after comparing the iPS cells of the different origin cell, iPS cells derived from iris had better the cell shape and maintenance culture as nerve cells.

Free Research Field

分子細胞生物学（眼科・再生）