2016 Fiscal Year Final Research Report
Pharmacological study on increased bone formation by alpha1-adrenoceptor signaling in bone metabolism.
| Project/Area Number |
26462827
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 交感神経 / 骨代謝 / α1B-アドレナリン受容体 / 骨形成 / 骨芽細胞 |
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| Outline of Final Research Achievements |
To obtain a better understanding of bone remodeling by the sympathetic nervous system, we investigated the role of alpha 1B-adrenoceptor signalling. The systemic administerion of prazosin, alpha1-adrenoceptor antagonist, decreased the bone formation in mice. In addition, alpha1B-adrenoceptor-deficient mice showed a lower bone mass due to decreased bone formation, without exhibiting any changes in bone-resorbing activity. Furthermore, the treatment of phenylephrine, alpha1-adrenoceptor agonist, with osteoblastic MC3T3-E1 cells increased the expression of the transcriptional factor CCAAT/enhncer-binding protein delta (Cebpd). The over-expression of Cebpd induced cellular proliferation in MC3T3-E1 cells, whereas the silencing of Cebpd suppressed it. These results suggested that alpha1B-adrenoceptor signalling is required for bone formation and regulated cellular proliferation through a mechanism relevant to the up-regulation of Cebpd in osteoblasts.
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| Free Research Field |
歯科薬理学
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