2016 Fiscal Year Final Research Report
Initial step of gravity sensing in cultured animal cells
| Project/Area Number |
26506009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
宇宙生命学
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 宇宙生命科学 / 微小重力 / 動物細胞 / 細胞力覚 |
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| Outline of Final Research Achievements |
Recent studies have cleared that individual cells can sense microgravity. However, this sensing mechanism remains largely unknown. We hypothesize that in response to altered gravity, the tension generated in SFs are altered, and that it cause gravity-dependent cellular responses. To verify it, we examined the substrate rigidity-dependent responses of mesenchymal stem cells (MSCs) under simulated microgravity. It is known that MSCs could change rigidity-dependently the tension in SFs to induce the rigidity-dependent responses. Actually at 1 g MSCs cultured on the soft substrate which could weaken the SF tension, decreased osteoblastic differentiation compared with those on the stiff substrate. However, the rigidity-dependent responses of MSCs were not found under simulated microgravity. These results suggest that stimulated microgravity could weaken the SF tension of MSCs even on the stiff substrate and that changes in the SF tension may mediate the gravity sensing of MSCs.
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| Free Research Field |
細胞生物物理学
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