Biocontainment of genetically modified bacterium by programed bacterial cell death.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26550066
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Modeling and technologies for environmental conservation and remediation
• Research Institution: Yamagata University
• Principal Investigator: HARA TOMIJIRO 山形大学, 理工学研究科, 教授 (70616193)
• Co-Investigator(Kenkyū-buntansha): 大場 好弘 山形大学, 理工学研究科, 教授 (60152237) ; 高塚 由美子 山形大学, 理工学研究科, 助教 (70570810)
• Research Collaborator: KATAKURA Sonoka ; FUJIOKA Tomoaki ; TAKENAKA Yasuhiro
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: コマモナス・テストステロニYAZ2株 / 分子育種 / 生物学的封じ込め / プログラム細菌細胞死 / 環境修復 / ポリ塩化ビフェニル

Outline of Final Research Achievements

We tried to create new bacterium by molecular bleeding approach in this research program. It was based on the whole genome sequence of Commamonas testosteroni YAZ2 strain (YAZ2 strain), which is a strong Polychlorinated biphenyls degrader. In particular we intended giving the ability of biocontainment to the new bacterium. Here, we report that YAZ2 strain has two replicons that has been designated one chromosome (5.45 Mbp) and one circular plasmid (0.9 Mbp), and It was analyzed by Next-generation DNA sequencing method and bio-informatics technology. In addition, we were took the genes associated with several bacterial cell death factor have been located on the replicons. On the other hand, based on the genes of YAZ2 strain, we have firstly succeeded to create new engineering Escherichia coli derivative strain that degrades PCB, as well as wild-type YAZ2 strain. Now, we are continuing to bleed engineered PCB degraders that have functional biocontainment.

Free Research Field

微生物学