2016 Fiscal Year Final Research Report
Elucidation of physical properties and chemical components of glue secretion used in breeding behavior of Breviceps frog and attempt at its artificial breeding
| Project/Area Number |
26560437
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
UKENA Kazuyoshi 広島大学, 総合科学研究科, 教授 (10304370)
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| Research Collaborator |
SAWADA Hitoshi
NAKAZAWA Shiori
HEMMI Keitaro
VENCES Miguel
MALONE John
MINTER Leslie
Du PREEZ Louis
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 生体機能物質 / 非モデル動物における生体物質の探索 / 人工繁殖 / 糊状物質 / プロテオーム / トランスクリプトーム |
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| Outline of Final Research Achievements |
We investigated the physical properties, chemical components, and candidate genes of the glue secration used in the reproduction activity in Breviceps frogs. As the results of this study, the adhesion strength of glue secration was about 500 g/cm2, and it was found that its main constituent is protein. It was suggested that there are four candidate proteins of the frog glue: three proteins similar to a non-glue skin-secrated protein known in another frog taxon and one novel protein.
We attempted at the artificial breeding of B. adspersus. As the result, it was revealed that a cactail of gonadotropin-releasing hormone agonist and dopamine antagonist, called Amphiplex, can induce the ovulation of this species. In addition, we succeeded to artificailly induce their amphiplexus and observe the nesting and spwning under the captive condition for the first time.
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| Free Research Field |
分子遺伝学・系統分類学
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