Development of an aquatic pest control with crystalline toxin modified cell recognition sites

2016 Fiscal Year Final Research Report

• Project/Area Number: 26660044
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Kindai University
• Principal Investigator: TAKEBE So 近畿大学, 生物理工学部, 教授 (20227052)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 微生物農薬 / 水生有害生物 / 選択毒性 / タンパク質不溶化 / Bacillus thuringiensis

Outline of Final Research Achievements

In order to control aquatic pests, a system for preparing an insoluble protein toxin was constructed by fusing a cell recognition site, a cytotoxic site and a protein crystallization tag. Insolubilizing increases the concentration of toxin, it is possible to cause a target organism to take a lethal dose. insolubilizing also suppress dilution and loss of toxin in water, and unnecessary environmental contamination. Cry46Ab derived from soil bacteria showed lethal activities against mosquito insters and the apple snail, but not against medaka. Therefore, insoluble toxin granules of Cry46Ab with a crystallization tag was prepared, and it was confirmed that this fusion protein retained lethal activities.

Free Research Field

分子生物学