2016 Fiscal Year Final Research Report
Simplification of lignin aromatic composition by bacterial lignan transformation systems
| Project/Area Number |
26660143
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
UTSUMI Ryutaro 近畿大学, 農学部, 教授 (20151912)
SHIBATA Daisuke かずさDNA研究所, 産業基盤開発研究部, 部長 (10370925)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | リグニン / リグナン / 芳香核 / 単純化 |
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| Outline of Final Research Achievements |
A human intestinal bacterium Blautia producta ATCC27340, whose draft genomic sequence has been released, demethylated two lignans, secoisolariciresinol and matairesinol. The bacterium demethylated secoisolariciresinol faster than matairesinol, and aromatic B-ring of matairesinol was preferentially demethylated. Analysis of draft genome of the bacterium revealed that the genomic sequence contained a cluster that encoded four proteins required for tetrahydrofolate-dependent demethylation of aromatic methoxyl groups. We obtained the complementary DNAs of the four proteins to set up lignan demethylation assay using the recombinant proteins.
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| Free Research Field |
植物代謝機能化学
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