" Selection of differentiated neural stem cells from iPS cells and removal of undifferentiated cells by using ultra-quick freezing with aquaporin expression "

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670146
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Keio University
• Principal Investigator: Yasui Masato 慶應義塾大学, 医学部, 教授 (90246637)
• Co-Investigator(Renkei-kenkyūsha): OKADA Yohei 愛知医科大学, 医学部, 准教授(特任) (30383714) ; KATO Yasuhiro 北京大学, 医学部(天士力微循環研究センター), 室長 (40398780)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アクアポリン４ / iPS細胞 / 神経幹細胞 / ES細胞

Outline of Final Research Achievements

Based on the finding that membrane damage caused by ultra-quick freezing is rescued by the expression of AQP4, we proposed a novel cell-selection method using ultra-quick freezing combined with AQP expression. In this project, we examined if the residual undifferentiated cells can be removed during differentiation from iPS cells to neural stem cells by the ultra-quick freezing since AQP4 expression is induced in neural stem cells. As expected, AQP4 positive cells were slightly concentrated. However, there was no difference, before and after the ultra-quick freezing, in terms of tumor formation after transferring of the neural stem cells into mouse testis tissues. Further optimal conditioning of AQP4 expression is required in neural stem cells for clinical application.

Free Research Field

薬理学