Establishing of Chlamydia phage mediated novel gene transfer system for Chlamydia pneumoniae.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670214
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: University of the Ryukyus
• Principal Investigator: Hirai Itaru 琉球大学, 医学部, 教授 (00359994)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 肺炎クラミジア / 組み換えファージシステム

Outline of Final Research Achievements

In this study, we tried to establish novel gene transfer system using a chlamydia phage phiCPAR39, which can infect Chlamydia pneumoniae. Chromosome of the chlamydia phage phiCPAR39, was cloned and utilized to construct a shuttle vector which can be replicated in both Chlamydia pneumoniae and Escherichia coli. To establish E. coli strain which produce recombinant chlamydia phage, expression of essential chlamydia phage proteins in E. coli cells were indispensable. However, expression of some of the chlamydia phage proteins were not confirmed. Consequently, E. coli strain, which would be utilized for production of the recombinant chlamydia phage, was not established.

Free Research Field

分子微生物学