Search and functional analysis of secreted microRNA associated with mitochondrial quality control

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670374
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General internal medicine(including psychosomatic medicine)
• Research Institution: Department of Clinical Research, National Hospital Organization Tokushima National Hospital
• Principal Investigator: Mitsui Takao 独立行政法人国立病院機構徳島病院(臨床研究部), その他部局等, その他 (80294726)
• Co-Investigator(Kenkyū-buntansha): MAKI YUKIKO (黒田 由紀子) 独立行政法人国立病院機構・徳島病院, 臨床研究部, 研究員 (70398014) ; KAWAMURA KAZUYUKI 独立行政法人国立病院機構・南京都病院, 臨床研究部, 臨床研究部長 (10450959)
• Co-Investigator(Renkei-kenkyūsha): KAJI RYUJI 徳島大学, 大学院臨床神経科学, 教授 (00214304)
• Research Collaborator: TAKAHASHI RYOSUKE ; FUJIMOTO MIKI ; SOGO MASAKO
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: パーキン / miRNA

Outline of Final Research Achievements

We have reported that the Parkin protein to promote transcription of mitochondrial genes.We hypothesized that Parkin is involved in the processing of miRNA associated with the mitochondria.In the present study, we investigated the role of Parkin in miRNA processing. We found that parkin is bonded to Drosha protein in the nuclear and mitochondrial fractions of cultured cells. This result suggests that Parkin to the involved miRNA processing via the Drosha complex in the nuclei and mitochondria. We have found that Parkin is bonded to miR132 and pre-miR132 in both fractions.

Free Research Field

免疫細胞学　神経内科学