2015 Fiscal Year Final Research Report
Establishment of virtual assay system by molecular dynamic simulation of HCV antiviral resistance
| Project/Area Number |
26670375
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Hokkaido University |
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Principal Investigator |
Sakamoto Naoya 北海道大学, 医学(系)研究科(研究院), 教授 (10334418)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO TOSHINORI 北海道大学, 情報科学研究科, 教授 (00323692)
SUDA GOKI 北海道大学, 医学研究科, 特任助教 (20447460)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | ウイルス学 / 肝炎ウイルス |
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| Outline of Final Research Achievements |
1. We conducted a molecular dynamic simulation of NS5A protein, its dimer and NS5A inhibitor (daclatasvir), and designated individual optimal 3D structures.
2. We conducted a molecular dynamic simulations of NS5A dimer and daclatasvir on the ER membrane.
3. We conducted an in-vitro assay by using HCV replicon system and confirmed expected effects of resistance-associated substitutions.
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| Free Research Field |
内科学
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