Assessment of micro residual disease with ultra deep sequencing

2015 Fiscal Year Final Research Report

• Project/Area Number: 26670492
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: National Center for Child Health and Development (2015); The University of Tokyo (2014)
• Principal Investigator: Kato Motohiro 国立研究開発法人国立成育医療研究センター, その他部局等, その他 (40708690)
• Co-Investigator(Renkei-kenkyūsha): Takita Junko 東京大学, 小児科, 准教授 (00359621)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 白血病 / バイオマーカー

Outline of Final Research Achievements

To improve “stratification strategy based on prognostic factors”, we planned to establish detection system of micro residual disease (miRD) with higher sensitivity compared to standard methods. First, we assessed sensitivity using virtual tumor specimen with serial dilution by next generation sequencer, and 10-3 to 10-4 of miRD could be detected. Using this method, by obtaining miRD status, we could illustrate dynamic evolutionary process of a case with unique coincidence of ALL and LCH. Furthermore, we performed miRD detection using droplet digital PCR technology, and its sensitivity and quantitativity.

Free Research Field

小児血液・腫瘍学