Assessment of genotoxic agents by using recombination-associated protein-deficient cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26740026
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Hiroshima University
• Principal Investigator: Suzuki Tetsuya 広島大学, 医歯薬保健学研究院(薬), 助教 (20573950)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: DNA二本鎖切断 / 相同組換え / DNAヘリカーゼ / DNAリゾルベース

Outline of Final Research Achievements

DNA helicases and DNA resolvases are believed to play important roles in DNA strand break repair. In this study, we established cell lines deficient in a DNA helicase (WRN/RECQL5) or a DNA resolvase (GEN1/SLX4) and examined their cytotoxic sensitivities toward various genotoxic agents.
WRN- and RECQL5-deficient cells displayed higher sensitivity to hydroxyurea than wild-type cells. GEN1-deficient cells displayed higher sensitivity to topoisomerase inhibitors than wild-type cells. SLX4-deficient cells exhibited higher sensitivity to alkylating agents. These results suggest that the knockout of each protein have cytotoxic effects on various genotoxic agents and the proteins may play important role for the practical threshold for genotoxicity.

Free Research Field

DNA修復