Direct study of neurotransmitter/Action potential correlation with a CMOS integrated carbon nanotube sensors arrays

2015 Fiscal Year Final Research Report

• Project/Area Number: 26790035
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Seichepine Florent 国立研究開発法人理化学研究所, 生命システムセンター, 特別研究員 (20650641)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: Neurotransmitter / CMOS / Microelectrode Array / Carbon nanotube / sensors

Outline of Final Research Achievements

We have created a high-density array of carbon nanotube field effect transistor sensors (CNT-FET) and we have proved that they can be used for the sensing of neurotransmitters after functionalization with specific enzyme. We have demonstrated the possibility of developing culture of neurons on those sensors and we have successfully records under those conditions. More specifically, we have created new type of CNT-FET sensors using electric field manipulation with yield of about 80% of small bundle on 1024 devices. By using liquid gate configuration, we have proved the high-sensitivity of our sensors. We have use non-covalent functionalization methods to bind glutamate oxidase to CNT-FET and we have shown that such biosensors are sensitive to glutamate. This accomplishment represents the fabrication of a new tool to investigate chemical release in culture medium which can be co-integrated with electrical recording.

Free Research Field

Nanobiotechnology