Analysis of nuclear and cytoskeletal dynamics underlying neuronal migration

2016 Fiscal Year Final Research Report

• Project/Area Number: 26830012
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Kyoto University
• Principal Investigator: Umeshima Hiroki 京都大学, 物質－細胞統合システム拠点, 研究員 (40525375)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 神経発生 / 細胞移動

Outline of Final Research Achievements

I performed time-lapse imaging of nuclear translocation during neuronal migration with a high-speed confocal microscope, in order to understand molecular mechanisms of neuronal migration. As a result, I found that the nucleus is deformed and rotated during neuronal migration. Moreover, molecular biological approaches suggested that force generated by microbule-based motor proteins drives both nuclear translocation and rotation of migrating neurons (submitting a manuscript for publication). On the other hand, I also performed traction force microscope analysis for migrating neurons and found traction force accompanied by nuclear translocation, which is generated by actomyosin contractile activity (preparing a manucscript).

Free Research Field

神経発生生物学