Estimating mechanical properties of moving cell sheets

2016 Fiscal Year Final Research Report

• Project/Area Number: 26840077
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: Kondo Yohei 京都大学, 情報学研究科, 特定助教 (00724444)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 組織力学 / 逆問題 / 時系列解析

Outline of Final Research Achievements

Measurement of biophysical parameters of living tissues such as rigidity often requires exogenous manipulation on the tissues, but such a manipulation perturbs spontaneous morphological processes undesirably. Here, we developed a non-invasive statistical method to estimate biophysical parameters. Our strategy is to model the tissue as a continuum mechanical system, and to use time-lapse imaging data of tissue dynamics in order to compute the maximum likelihood estimates of model parameters. We validated our method on epithelial spreading of Madin-Darby canine kidney cells, a well-known model system for collective migration.

Free Research Field

生物物理学