2015 Fiscal Year Final Research Report
Structure and function of chitinase-like proteins involved in cell wall biosynthesis.
| Project/Area Number |
26850066
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | chitinase-like protein |
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| Outline of Final Research Achievements |
AtCTL1, AtCTL2 and OsCTL1 are CTL, chitinase-like, proteins from Arabidopsis and rice (Oryza sativa). They consist of an N-terminal signal sequence, transmembrane region, GH19 chitinase-like domain, and C-teminal extension. We expressed the GH19 chitinase-like domains of AtCTl1 and AtCTL2 as fusion protein with glutathione S-transferase and SUMO protein, respectively, and purified them to homogeneity as judged by SDS/PAGE.
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| Free Research Field |
生化学
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