Correlation analysis between glycosylation and the efficacy of antibody preparation in human blood

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Kyoto University
• Principal Investigator: TSUDA Masahiro 京都大学, 薬学研究科(研究院), 講師 (10726813)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 抗体 / 糖鎖 / 癌 / 質量分析 / バイオテクノロジー / 薬物動態 / 血中濃度 / 翻訳後修飾

Outline of Final Research Achievements

In current study, we analyzed the carbohydrate structure of anti-CD20 monoclonal antibody rituximab to evaluate effect of glycosylation on the efficacy and the pharmacokinetics of antibody preparation. Several glycan species were identified in rituximab. The relative content of glycoforms changed in a time-dependent mannar. These changes affected to pharmacokinetics of rituximab. In conclusion, we obtained novel findings in the role of carbohydrate of rituximab.

Free Research Field

医療薬剤学