The mechanism for activation of a spinal microglia induced by multiple stress loading

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860381
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: Nagoya University
• Principal Investigator: YASUI MASAYA 名古屋大学, 全学技術センター(医学), 技術専門職員 (10723695)
• Research Collaborator: KIYAMA Hiroshi ; MIZUMURA Kazue ; TOKIZANE Kyohei ; MENJO Yuki ; YOSHIMURA Takashi
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ストレス / 疼痛 / ミクログリア / 線維筋痛症 / 慢性疲労症候群

Outline of Final Research Achievements

The present study addressed whether the nonphysiological pain was occurred in different two　stress models. In the result, we demonstrated that CFS and FM models elicited mechanical allodynia and muscular hyperalgesia. Furthermore no signs of inflammation and injury incidents were observed in both the plantar skin and leg muscles. However, microglial accumulation and activation were observed in L4-L6 dorsal horn of CFS and FM model rats. To evaluate an implication of microglia in pain, minocycline was intrathecally administrated in CFS model rats. Minocycline significantly attenuated continuous multiple stresses induced mechanical hyperalgesia and allodynia. These results indicated that activated microglia were involved in the development of abnormal pain in CFS animals, suggesting that the pain observed in CFS and FM patients may be partly caused by a mechanism in which microglial activation is involved.

Free Research Field

疼痛学