Effect of alternative polyadenylation during epithelial-mesenchymal transition on cancer cell growth

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860509
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: The University of Tokushima
• Principal Investigator: NISHIDA Kensei 徳島大学, 大学院医歯薬学研究部, 准教授 (10624033)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 選択的ポリアデニレーション制御

Outline of Final Research Achievements

To investigate the effect of alternative polyadenylation during epithelial-mesenchymal transition (EMT) on the HCT116 colon cancer cell line, we succeeded to select a population of cells capable of migrating. Pathway analysis was performed using Ingenuity pathway analysis (IPA) software. IPA revealed that VIM, ZEB1 and FOXQ1, which are EMT marker genes, were upregulated in the selected cells. We identified the genes whose 3’UTR are shorten in the selected cells using Exon Array. Furthermore, we found that protein levels of CPSF family were increased in the highly migrated cells. These data suggested that regulation of CPSF family protein levels alters the ability of migration through changing 3'UTR length of the certain genes.

Free Research Field

RNAバイオロジー