2015 Fiscal Year Final Research Report
Search for pathogenesis and novel therapeutics of acquired myelodysplastic syndromes using reprogramming technology
| Project/Area Number |
26860727
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 骨髄異形成症候群 / 血液分化異常 / リプログラミング / iPS細胞 / 病態解明 / 新規治療薬 |
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| Outline of Final Research Achievements |
We successfully generated multiple iPS cell lines from MDS clones (MDS-iPSC lines) of several MDS patients with abnormal karyotypes. We assessed hematopoietic differentiation potential of MDS-iPSC lines and isogenic normal T-iPSC lines. Hematopoietic colony formation in methylcellulose culture and further differentiation in erythroid and neutrophil culture were severely impaired in all tested MDS-iPSC lines.
Next, we performed microarray analysis in hematopoietic progenitor cells re-induced from MDS-iPSC lines and isogenic normal iPSC lines, identifying MDS-specific expression changes.
Finally, we performed whole-exome analysis to find that some of the somatic mutations detected in bulk cells of this MDS patient are shared in MDS-iPSC lines but not in isogenic normal iPSC lines.
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| Free Research Field |
血液腫瘍学、幹細胞生物学
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