Characterization of sevoflurane effects on Per2 expression using ex vivo bioluminescence imaging of the suprachiasmatic in transgenic rats

2015 Fiscal Year Final Research Report

• Project/Area Number: 26861252
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: Nippon Medical School
• Principal Investigator: Anzai Megumi 日本医科大学, 医学部, 助教 (30719000)
• Co-Investigator(Renkei-kenkyūsha): IIJIMA Norio 日本医科大学, 医学部, 准教授 (00285248) ; SAKAMOTO Atsuhiro 日本医科大学, 大学院医学研究科, 教授 (30196084) ; OZAWA Hitoshi 日本医科大学, 大学院医学研究科, 教授 (60169290) ; HIGO Shinpei 日本医科大学, 医学部, 助教 (50623922) ; TAKUMI Ken 日本医科大学, 医学部, 助教 (40553269)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 時計遺伝子 / Per2 / sevoflurane / phase-shift / 概日リズム / GABA受容体 / 株細胞

Outline of Final Research Achievements

Remarkable and reversible suppression of Per2 mRNA expression in the suprachiasmatic nucleus (SCN) by inhalation anesthetics is considered as an biochemical marker of the anesthetic effect. This project aims to elucidate the mechanism of inhalation anesthetics paying attention to this suppressive effect.1) We adopted a imaging system for SCN slices derived from Per2-dLuc transgenic rats. This system was enable us to examine the intra-SCN regional specificity, time-dependency, and pharmacological basis of sevoflurane-effects. Pharmacological experiments revealed that GABAergic signals played important roles in the anesthetic effect.
2) The SCN contains various types of neurons, and this complexity makes it difficult to investigate the molecular mechanisms of anesthesia. Here, we established an in vitro experimental system using a cell line、GT1-7, which will open up the possibility to investigate more precisely the mechanisms underlying anesthetic action.

Free Research Field

麻酔科学