Analysis of the mechanisms for the inflammatory bone resorption by an osteoclasts differentiation inhibitory peptide derived from mesenchymal stem cells.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26893249
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: Kikuchi Emiko (青松恵美子) 岩手医科大学, 歯学部, 研究員 (50733854)
• Project Period (FY): 2014-08-29 – 2016-03-31
• Keywords: 間葉系幹細胞 / 破骨細胞 / 炎症性骨吸収

Outline of Final Research Achievements

In the present study, we have examined the mechanism for osteoclast differentiation and inflammatory bone resorption by an osteoclasts differentiation inhibitory peptide, scrapie-responsive gene 1 (SCRG1), derived from mesenchymal stem cells (MSC). Mouse macrophage-like Raw264.7 cells as the osteoclast precursor were investigated activation of the intracellular signal transduction pathways and gene expression analysis after treatment with recombinant mouse SCRG1 (rmSCRG1). As a result, rmSCRG1 was significantly enhanced the phosphorylation of ERK1/2. In addition, mrSCRG1 was promoted the expression of chemokine receptor, CCR7, not only reduced the expression of LPS-induced chemokines, CCL22. Therefore, these results strongly suggested that SCRG1 secreted from MSC in the inflammatory tissues suppress the proinframmation and inflammatory bone resorption through a receptor complex, BST-1/β-integrin on the cell surface and activation of ERK1/2 of macrophages.

Free Research Field

歯科矯正学