1986 Fiscal Year Final Research Report Summary
An in vitro Method for Testing the Sensitivity to Drugs of Human Malignant Bone and Soft Tissue Tumor
| Project/Area Number |
59440063
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | SAPPORO MEDICAL COLLEGE |
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Principal Investigator |
ISHII SEIICHI SAPPORO MEDICAL COLLEGE, Proffesor, 医学部, 教授 (20001000)
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| Co-Investigator(Kenkyū-buntansha) |
KOIWAI SOICHIRO SAPPORO MEDICAL COLLEGE, Associate Proffesor, 放射線医学, 講師 (90045336)
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| Project Period (FY) |
1984 – 1986
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| Keywords | HUMAN RHABDOMYOSARCOMA / OSTEOSARCOMA / NUDE MOUSE / TISSUE CULTURE / AUTORADIOGRAPHIC STUDIES / 核酸標識化合物 / 核酸合成抑制効果 |
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| Research Abstract |
Sensitivity test to anticancer drugs by mesuring the supression of nucleic acid synthesis is considered to be useful in clinical application. We have developed new test system in which sensitivity is investigated with cultured cells from transplanted tumors of original sarcomas onto the back of nude mice. Results are as follows.
(1) We examined sensitivity of anticancer drugs in 7 human rhabdomyosarcomas and 12 human osteosarcomas. The results of sensitivity to drugs were obtained in about 70 percent of all of the trials made for our sarcoma cases.
(2) Rhabdomyosarcomas showed a tendency to be sensitive to VCR, MMC, ADM, BLM MTX, ADM, MMC, CPM, ACT-D are estimated as high sensitive drugs to osteosarcomas.
(3) We examined the reliability of the sensitivity test using <^3H> -UdR, <^3H> -TdR and <^(14)C> -formate. Triciated UdR was considered to be the most appropriate labeled precursor that can show accurately the degree of supression of nucleic acid synthesis.
(4) The results of sensitivity
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were somewhat comparable with those achieved by mesuring cell survival. The drugs inhibited the regrowth of cultured cells from rhabdomyosarcomas and osteosarcomas completely, when the ratio of supression of nucleic acid synthesis caused by these drugs showed more than 75 percent.
(5) The results of sensitivity to anticancer drugs were also comparable with those achieved by mesuring the success ratio of back transplantation of rhabdomyosarcomas, and of lung metastasis of osteosarcoma onto nude mice after treatment with drugs which showed sensitive in our test system.
(6) We investigated whether the results of sensitivity showed the reproducibility or not, by repeating the sensitivity test 2 to 7 times over during 2 months for each osteosarcoma case. 6 out of 8 osteosarcomas showed same sensitivity pattern to anticancer drugs in each test we repeated.
(7) The correlation between sensitivity testing and clinical results was examined, showing true positive rate: 50 percent and true negative rate: 66.6 percent, when tested by mesuring the supression of nucleic acid synthesis. We concluded our sensitivity test has a significance when we want to know non-effective anticancer drug in clinical use. Less
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