1986 Fiscal Year Final Research Report Summary
Mechanisms of somatic embryogenesin in plant suspension cultures
| Project/Area Number |
60440003
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | TOHOKU UNIVERSITY
Tokyo Institute of Technology |
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Principal Investigator |
KOMAMINE Atsushi Biological Inst., Faculty of Science, Tohoku Univ., Professor, 理学部, 教授 (90011494)
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| Project Period (FY) |
1985 – 1987
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| Keywords | Carrot / Cell Culture / Microinjection / Somatic Embryogenesis / Tiプラスミド / ニンジン / 培養細胞 / 分化の決定 |
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| Research Abstract |
A high frequency synchronous somatic embryogenesis from single cells was estalished in carrot suspension cultures. The following results were obtained using this system.
1. Polarities of cell division activity, DNA and RNA synthesis, mRNA and calcium localization were observed during the process from single cells to embryogenic cell clusters. The apperance of these polarities are closely associated with embryogenesis, suggesting that it may play important roles in determination of somatic embryogenesis.
2. The technique of microinjection to single cells was established. Seventy % of microinjected cells could divide and 50 % could differentiate to embryos. DNA fragments from Ti plasmid as a foreign DNA was microinjected to single cells and it was confirmed that introduced foreign DNA was replicated and expressed after differentiation to embryos at 5 % frequency of transformation.
3. Specific protein for embryogenesis was detected. Microinjection of the monoclonal antibody for the protein to single cells inhibited embryogenesis, indicating that the protein may play a critical role in embryogenesis.
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