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1987 Fiscal Year Final Research Report Summary

The Physiological Role of D-Amino Acid Oxidase in the Mammalian Central Nervous System

Research Project

Project/Area Number 60580150
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 代謝生物化学
Research InstitutionShiga University of Medical Science

Principal Investigator

HORIIKE Kihachiro  Shiga University of Medical Science, Associate Professor, 医学部, 助教授 (80089870)

Co-Investigator(Kenkyū-buntansha) ISHIDA Tesuo  Shiga University of Medical Science, Assistant Professor, 医学部, 助手 (10176191)
Project Period (FY) 1985 – 1987
KeywordsD-Amino acid oxidase / Flavoenzyme / Central nervous system / Thiazolidine-2-carboxylate / Coupled peroxidation method / Neuroglia / Astrocyte
Research Abstract

To elucidate the physiological role of D-amino acid oxidase (DAO), a flavin-containing peroxisomal enzyme, in the mammalian central nervous system, it isimportant to explore the histological localization of DAO and its correlation to the oxidizing activity of thiazolidine-2-carboxylate (TC), which is an adduct of cysteamine and glyoxylate and is believed to be a physiological substrate for DAO.
Using serial fixed sections, we carried out a histochemical study on DAO in the whole brain of rat by a sensitive peroxidase-coupled procedure which we devised for histochemical detection of DAO activity. This method is based on the intensifying effect of nickel ions on diaminobenzidinebased product formation with peroxidase. DAO activity was found to be localized exclusively in the lower brain stem, cerebellum and spinal cord, and to be present only in astrocytes and Bergmann glial cells, but not in neuronal components, endothelial cells or ependymal cells. Form electron microscopic observations, the greater proportion of DAO activity was present in the glial spaces around various kinds of synapses. We also applied the above technique for the detection of TC oxidase activity. The localizations of this oxidase activity in the kidney, liver and brain of rat were in accord with those of DAO. On the other hand, the activity of D-aspartate oxidase, another peroxisomal flavoenzyme, was found in the endbrain in which DAO activity was not detectable.
The above results clearly demonstrate the regional differentiation of astrocytes and the uneven distribution of peroxisomal enzymes in the brain. It is also suggested the possibility that DAO is involved in the metabolic regulation by controlling the intracellular level of cysteamine and its metabolite, an oxalyl thiol ester, which are known to be metabolic effectors.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Hiromasa Tojo: J.Biol.Chem.260. 12607-12614 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiromasa Tojo: J.Biol.Chem.260. 12615-12621 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kihachiro Horiike: Acta Histochem.Cytochem.18. 539-550 (1985)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kihachiro Horiike: Brain Res.Bull.19. 587-596 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tetsuo Ishida: J.Theor.Biol.(1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] D.B.McCormick and D.E.Edmondson(eds.)Kihachiro Horiike: "Flavins and Flavoproteins" (Walter de Gruyter & Co.Berlin・New York), (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiromasa Tojo: "Self-association mode of a flavoenzyme D-amino acid oxidase from hog kidney. I. Analysis of apparent weight-average molecular weight data for the apoenzyme in terms of models." J. Biol. Chem.260. 12607-12614 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiromasa Tojo: "Self-association mode of a flavoenzyme D-amino acid oxidase from hog kidney. II. Stoichiometry of holoenzyme association and energetics of subunit association." J. Biol. Chem.260. 12615-12621 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kihachiro Horiike: "Histochemical staining of cells containing flavoenzyme D-amino acid oxidase based on its enzymatic activity: Application of a coupled peroxidation method." Acta Histochem. Cytochem.18. 539-550 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kihachiro Horiike: "Localization of D-amino acid oxidase in Bergmann glial cells and astrocytes of rat cerebellum." Brain Res. Bull.19. 587-596 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuo Ishida: "Interaction of protein with a self-associating ligand. Deviation from a hyperbolic binding curve and the appearance of apparent co-operativity in the Scatchard plot." J. Theor. Biol.(1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kihachiro Horiike: Flavins and Flavoproteins (D. B. McCormick and D. E. Edmondson, eds.). Walter de Gruyter & Co., Berlin. New York, (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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