1988 Fiscal Year Final Research Report Summary
Development of Precise Aiming System of Laser Microbeam Cell Micromanipulation
| Project/Area Number |
62870072
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
YAMAMOTO Hajime Tokyo Medical and Dental University, Professor, 歯学部, 教授 (60005014)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAYANO Teruo Tokyo Medical and Dental University, Lecturer, 歯学部, 講師 (90152884)
|
|---|
| Project Period (FY) |
1987 – 1988
|
| Keywords | Laser / Micromanipulation / Bioengineerning / Cell biology |
|---|
| Research Abstract |
A novel biotechnique of cell micromanipulation by laser microbeam is pioneered. The system was essentially an inverted microscope with a thermostat interfaced to a He-Ne laser, a Ar' laser, a Nd:YAG laser and other kinds of laser, employed for microbeam cell irradiation, Each laser beam was guided by flexible optical fiber which could connect between several laser apparatus, microscope and laser beam selector. By a laser beam selector, the desired laser was selected to be irradiated to the specimen. The phase-contrasted image of the specimen was visually observed on a TV monitor screen and registered in a video tape recorder and a frame memory for a long time after the laser irradiation. The focal spot of the laser through a dichromatic mirror was superimposed on the specimen image and the focused irradiation at any desired site of the specimen was given by moving mechanical stage. To get a good aim at the intracellular target, such as one of organellae, the pahse-contrasted image of the cells was computerized by an image processor and was visually observed on a monitor screen. Laser microbeam micromanipulation was designed on a monitor screen by a interactive computer system with a mouse input. The laser beam was guided to the shtter controller which released the shutter of the laser for given period of time. The focused laser spot size of 10 mum in diameter was available by a 10-fold objective. The mechanical stge movement was accomplished by a prezonelectric motor which was good for a ultramicromovement.
The present system of laser microbeam cell vaicromanipulation is probably more useful for studying the effects of laser irradiation on various type of cells than the in vivo systems. And our system has many advantages for detailed investigation of the effects on laser irradiation on the subcellular level of organella.
|
