1989 Fiscal Year Final Research Report Summary
Inactivation of Cu,Zn-superoxide dismutase due to glycation
| Project/Area Number |
63480501
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | Osaka University Medical School |
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Principal Investigator |
TANIGUCHI Naoyuki Osaka University Medical School Professor, 医学部, 教授 (90002188)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Toshihiro Osaka University Medical School Associate Professor, 医学部, 助教授 (00127242)
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| Project Period (FY) |
1988 – 1989
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| Keywords | superoxide dismutase / Cu,Zn-SOD / glycation / active site liganding loop / Enzyme-linked immunosorbent Assay / retinopathy |
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| Research Abstract |
Human erythrocyte Cu,zn-superoxide dismutase comprises both glycated and non-glycated forms. In the cases of crude extracts as well as pure enzyme preparation obtained from erythrocytes of patients with Werner's syndrome, the glycated form was found in the bound fraction with a boronate column as in normal controls. However, the specific activity of the glycated form expressed as units per mg Cu,Zn-superoxide dismutase protein was significantly low and the enzyme was unstable as compared to the normal form. We conclude that in Werner's syndrome there is an increased amount of the inactive glycated form. We also found that glycation of Cu,Zn-SOD is highly associated with diabetic complication such as diabetic rdtinopathy or diabetic cataract.
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[Publications] Taniguchi, N., Kinoshita, N., Arai, K., Iizuka, S., Usui, M., and Naito, T.: "Inactivation of erythrocyte Cu,Zn-superoxide dismutase through nonenzymatic glycosylation." The Maillard Reaction in Aging, Diabetes, and Nutrition. Alan R. Liss, Inc. 277-290, 1989.
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[Publications] Kawaguchi, T., Noji, S., Uda, T., Nakashima, Y., Takeyasu, A., Kawai, Y., Takagi, H, Tohyama, M., and Taniguchi, N.: "A monoclonal antibody against COOH-terminal peptide of human liver manganese superoxide dismutase" J. Biol. Chem. 264, 5762-5767, 1989.
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「研究成果報告書概要(欧文)」より
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[Publications] Ishikawa, M., Yaginuma, Y., Hayashi, H., Shimizu, T., Endo, Y., and Taniguchi, N.: "Reactivity of a monoclonal antibody against manganese superoxide dismutase with human ovarian carcinoma." Cancer Res. 1990.
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「研究成果報告書概要(欧文)」より
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[Publications] Kawaguchi, T., Suzuki, K., Matsuda, Y., Nishiura, T., Uda, T., Ono, M., Sekiya, C., Ishikawa, M., Iino, S., Endo, Y., and Taniguchi, N.: "Serum Mn-superoxide dismutase: Normal values and increased levels in patients with acute myocardial infarction and several malignant diseases determined by enzyme-linked immunosorbent assay using a monoclonal antibody." J. Immunol. Methods, 127 249-254, 1990.
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「研究成果報告書概要(欧文)」より
