1989 Fiscal Year Final Research Report Summary
Analysis of neuronal network disturbance and trial of active regeneration by transplantation
| Project/Area Number |
63570679
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
YAMADA Kazuo Osaka University Medical School Neurosurgery Assistant Professor, 医学部, 助手 (90150341)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Toru Osaka University Medical School Associate Professor, 医学部, 助教授 (20135700)
MOGAMI Heitaro Osaka University Medical School Professor, 医学部, 教授 (00028309)
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| Project Period (FY) |
1988 – 1989
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| Keywords | ischemic neuronal death / retrograde degeneration / neuronal network / ischemic axonal damage / neurotrophic factors / nerve growth factor / epidermal growth factor / basic fibroblast growth factor |
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| Research Abstract |
We have analyzed the tissue damage after experimental ischemic infarction, and clarified that damage extend outside of the infarction through neuronal network, i.e. anterograde degeneration and retrograde degeneration. There also present functionally isolated area adjacent to the infarction, and this area has to be supported for regeneration. We have delineated some neurotrophic factors located in this area. The factors support survival and neurite extension of the cultured fetal neurons, and their molecular weight was around 8kD and 22kD by gel filtration. These factors were probably produced by c-fos positive reactive astrocytes located in this area. We tested whether neurotrophic factors modifies retrograde thalainic degeneration caused by cortical infarction. We tested recombinant basic fibroblast growth factor (bFGF), which was given intracisternally one day after infarction and repeated every week for 4 weeks. The bFGF partially reversed thalamic atrophy caused by cortical infarction (bFGF: thalamic area was 92% of the contralateral side; vehicle group: thalamic area was 79% of the contralateral side, p<0.05). The bFGF group showed reactive astrocytes located periventricular white matter suggesting that bFGF affects astrocytes to produce some factors which prevent retrograde,degeneration of the thalamus after cortical infarction.
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