1989 Fiscal Year Final Research Report Summary
Analysis of virus-host interaction in multiplication of tobacco mosaic virus
| Project/Area Number |
63580201
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | University of Tokyo |
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Principal Investigator |
MESHI Tetsuo Univ. Tokyo, Fac. Science, Research Associate, 理学部, 助手 (40157813)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Yuichiro Univ. Tokyo, Fac. Science, Research Associate, 理学部, 助手 (60183125)
OKADA Yoshimi Univ. Teikyo, Fac. Science & Technology, Professor, 理工学部, 教授 (30011703)
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| Project Period (FY) |
1988 – 1989
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| Keywords | tobacco mosaic virus / Tm-1 gene / Tm-2 gene / in vitro transcription / replication / cell-to-cell movement / resistance reaction / nucleotide sequence |
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| Research Abstract |
Resistance-breaking strains named Lta1 and Ltb1 can overcome the resistance conferred by the Tm-1 and Tm-2 genes in tomato, respectively. To understand the resistance mechanisms and to get hints for normal process of multiplication of tobacco mosaic virus (TMV), mutations of Lta1 and Ltb1 responsible for overcoming the resistance were determined.
From the nucleotide sequence comparison and subsequent reverse- genetic analysis, It was found that two base substitutions in the 130k/180k protein genes resulting in amino acid changes from Gln(979) to Glu and from His(984) to Tyr are important for the ability to overcome the Tm-1 resistance. Further analysis of other resistance-breaking strains and pseudorevertants suggested that an electrostatic interaction is involved in the resistance mechanism. Analysis in protoplasts supported that the resistance works in the course of replication. This is consistent with the involvement of the 130k and 180k proteins in replication.
In the case of Ltb1, it was found that two base substitutions in the 30k protein gene resulting in amino acid changes from Cys(868) to Phe and from Glu(133) to Lys are involved in the property to overcome the Tm-2 resistance. Since the 30k protein is known to be involved in cell-to-cell movement, and since the mutations occur in well-conserved regions in the 30k protein, the Tm-2 gene would seem to block the normal cell-to-cell movement.
Analysis using in vitro generated mutants revealed that the coat protein gene encodes the determinant for induction of the hypersensitive response in plants with the N' gene. It is not necessary to be assembled into virus particles.
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Research Products
(10 results)
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[Publications] Meshi, T., Motoyoshi, F., Adachi, A., Watanabe, Y., Takamatsu, N., and Okada, Y.: "Two concomitant base substitutions in the putative replicase genes of tobacco mosaic virus confer the ability to overcome the effects of a tomato resistance gene, Tm-1." EMBO J., vol. 7, pp.1575-1581, (1988).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamaya, J., Yoshioka, M., Meshi, T., Okada, Y., and Ohno, T.: "Expression of tobacco mosaic virus RNA in transgenic plants." Mol. Gen. Genet., vol. 211, pp.520-525, (1988).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamaya, J., Yoshioka, M., Meshi, T., Okada, Y., and Ohno, T.: "Cross protection in transgenic plants expressing a mild strain of tobacco mosaic virus." Mol. Gen. Genet., vol. 215, pp.173-175 (1988).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Meshi, T., Motoyoshi, F., Maeda, T., Yoshiwoka, S., Watanabe, H., and Okada, Y.: "Mutations in the tobacco mosaic virus 30-kD protein gene overcome Tm-2 resistance in tomato." Plant Cell, vol 1, pp.515-522 (1989).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Saito, T., Yamanaka, K., Watanabe, Y., Takamatsu, N., Meshi, T., and Okada, Y.: "Mutational analysis of the coat protein gene of tobacco mosaic virus in relation to hypersensitive response in tobacco plants with the N' gene." Virology, vol. 173, pp.11-20 (1989).
Description
「研究成果報告書概要(欧文)」より
