Role of DNA topology in gene expression.

2020 Fiscal Year Annual Research Report

• Project Area: Chromatin potential for gene regulation
• Project/Area Number: 19H05261
• Research Institution: Kyoto University
• Principal Investigator: Canela Andres 京都大学, 白眉センター, 特定准教授 (90837585)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: DNA Topoisomerases / Transcription / DNA torsion / RNA polymerase II / Chromatin loop

Outline of Annual Research Achievements

During the fiscal year 2020, I evaluated the impact of torsional stress in transcription using cell lines deficient for DNA topoisomerase 2 (TOP2α and TOP2β) generated in the fiscal year 2019. Combining nascent RNA FISH with ChIP-seq for PolII I quantified how the torsional stress accumulated in absence of topoisomerase 2 activity affects transcription dynamics. I measured transcription bursting, intensity of transcription and rates of PollII binding and pause release

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] END-seq: An Unbiased, High-Resolution, and Genome-Wide Approach to Map DNA Double-Strand Breaks and Resection in Human Cells (2021)
  • Author(s): Wong N, John S, Nussenzweig A, Canela A.
  • Journal Title: Methods in Molecular Biology Volume: 2153 Pages: 9-31
  • DOI: 10.1007/978-1-0716-0644-5_2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes (2021)
  • Author(s): Huang SN, Michaels SA, Mitchell BB, Majdalani N, Vanden Broeck A, Canela A, Tse-Dinh YC, Lamour V, Pommier Y.
  • Journal Title: Science Advances Volume: 7 Pages: not yet
  • DOI: 10.1126/sciadv.abe0384
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Neuronal enhancers are hotspots for DNA single-strand break repair. (2021)
  • Author(s): Wu W, Hill SE, Nathan WJ, Paiano J, Callen E, Wang D, Shinoda K, van Wietmarschen N, Colon-Mercado JM, Zong D, De Pace R, Shih HY, Coon S, Parsadanian M, Pavani R, Hanzlikova H, Park S, Jung SK, McHugh PJ, Canela A, Chen C, Casellas R, Caldecott KW, Ward ME, Nussenzweig A.
  • Journal Title: Nature Volume: not yet (online) Pages: not yet
  • DOI: 10.1038/s41586-021-03468-5
  • Peer Reviewed / Int'l Joint Research