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2023 Fiscal Year Annual Research Report

Bottom-up creation of cell-free molecular systems by photophyscal methods

Publicly Offered Research

Project AreaBottom-up creation of cell-free molecular systems: surpassing nature
Project/Area Number 22H05423
Research InstitutionOsaka University

Principal Investigator

吉川 洋史  大阪大学, 大学院工学研究科, 教授 (50551173)

Project Period (FY) 2022-06-16 – 2024-03-31
Keywordsレーザー操作 / レーザー加工 / 超越分子システム / タンパク質 / 生体分子 / ソフトマター / 拡散 / 相分離
Outline of Annual Research Achievements

本研究では、研究代表者が得意とするレーザーの物理作用(光電場、光熱作用など)に基づく分子の集合・配列制御法を駆使し、得意な構造・機能を有する様々な無細胞分子システムをボトムアップ構築することにある。
昨年度に引き続き2023年度は、物理作用に基づくレーザー技術(例:レーザーアブレーション、レーザートラッピング)の高度化・先鋭化と、本学術変革領域で対象とする様々な試料を用いた共同研究に取り組んだ。レーザー技術の高度化・先鋭化については、様々な試料の結晶化をモデルとして、分子システムのボトムアップ構築に必要な核発生、結晶種形成、多形相転移などを時空間制御することに成功した。またその集合・配列プロセスを透過光・蛍光・偏光・ラマン散乱など多角的にとらえることで、ボトムアップ構築のダイナミクスや形成した分子システムの機能をその場評価できることを示した。特に2023年度は偏光干渉計測を重点的に行い、その新規超解像イメージングなどを開発することにも成功している。また領域代表の松浦(東工大)をはじめ、様々な領域内共同研究を展開した。その結果、タンパク質・核酸・低分子化合物など、分子量が大きく異なる様々な物質を局所濃縮可能であるという、超越分子システムの新規ボトムアップ構築法に関する重要な知見を得た。以上の成果はJ Phys Chem Lett誌など複数の原著論文として報告し、さらに様々な学会・シンポジウムなどでも発表した。

Research Progress Status

令和5年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和5年度が最終年度であるため、記入しない。

  • Research Products

    (32 results)

All 2024 2023 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (24 results) (of which Int'l Joint Research: 11 results,  Invited: 2 results)

  • [Int'l Joint Research] シンシナティ小児病院/スタンフォード大学/カリフォルニア州立大学ロサンゼルス校(米国)

    • Country Name
      U.S.A.
    • Counterpart Institution
      シンシナティ小児病院/スタンフォード大学/カリフォルニア州立大学ロサンゼルス校
  • [Int'l Joint Research] 国立陽明交通大学(その他の国・地域(台湾))

    • Country Name
      その他の国・地域
    • Counterpart Institution
      国立陽明交通大学
  • [Journal Article] Spatiotemporal Control of Polymorphic Phase Transition of Glycine Crystals by Three-Dimensional Femtosecond Laser Ablation Processing2024

    • Author(s)
      Hozumi Takahashi,Yudai Yoshimura,Ryota Murai,Ryuzo Kawamura,Mihoko Maruyama,Masahi Yoshimura,Yusuke Mori, Hiroshi Y. Yoshikawa
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 15 Pages: 180-186

    • DOI

      10.1021/acs.jpclett.3c02769

    • Peer Reviewed
  • [Journal Article] Advanced Interferometry with 3-D Structured Illumination Reveals the Surface Fine Structure of Complex Biospecimens2024

    • Author(s)
      Takahisa Matsuzaki, Ryuzo Kawamura, Akihisa Yamamoto, Hozumi Takahashi, Mai Fujii, Shodai Togo, Yosuke Yoneyama, Fumihiko Hakuno, Shin-Ichiro Takahashi, Masami Suganuma, Seiichiro Nakabayashi, Shivani Sharma, James K. Gimzewski, Hiroshi Y. Yoshikawa
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 15 Pages: 1097-1104

    • DOI

      10.1021/acs.jpclett.3c02767

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Spatiotemporal Control of Ice Crystallization in Supercooled Water via an Ultrashort Laser Impulse2023

    • Author(s)
      Hozumi Takahashi,Tatsuya Kono,Kosuke Sawada,Satoru Kumano,Yuka Tsuri,Mihoko Maruyama,Masashi Yoshimura,Daisuke Takahashi,Yukio Kawamura,Matsuo Uemura,Seiichiro Nakabayashi,Yusuke Mori,Yoichiroh Hosokawa,Hiroshi Y. Yoshikawa
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 14 Pages: 4394-4402

    • DOI

      10.1021/acs.jpclett.3c00414

    • Peer Reviewed
  • [Journal Article] 細胞接着の定量計測に基づく細胞組織の機能解明2023

    • Author(s)
      松﨑 賢寿, 吉川 洋史
    • Journal Title

      化学と生物

      Volume: 61 Pages: 269-273

    • Peer Reviewed
  • [Journal Article] Production of single crystalline seeds oforganic nonlinear optical materials via laser ablation2023

    • Author(s)
      Hozumi Takahashi,Megumi Shiraiwa,Valynn Katrine Mag-usara,Ruochen Dai,Verdad Agulto,Kosaku Kato,Makoto Nakajima,Mayu Yamaji,Seiichiro Nakabayashi,Mihoko Maruyama,Yusuke Mori,Masahi Yoshimura,Hiroshi Y. Yoshikawa
    • Journal Title

      The Journal of Physical Chemistry C

      Volume: 127 Pages: 14005-14012

    • DOI

      10.1021/acs.jpcc.3c03189

    • Peer Reviewed
  • [Journal Article] Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells2023

    • Author(s)
      Takahisa Matsuzaki,Yuma Kawano,Momoka Horikiri,Yuko Shimokawa,Takashi Yamazaki,Nao Okuma,Hiroyuki Koike,Masaki Kimura,Ryuzo Kawamura,Yosuke Yoneyama,Yasuro Furuichi,Fumihiko Hakuno,Shin-Ichiro Takahashi,Seiichiro Nakabayashi,Satoshi Okamoto,Hiromitsu Nakauchi,Hideki Taniguchi,Takanori Takebe,Hiroshi Y. Yoshikawa
    • Journal Title

      STAR Protocols

      Volume: 4 Pages: 102471

    • DOI

      10.1016/j.xpro.2023.102471

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Exploring Co-Crystallization Dynamics of L-Phenylalanine and Acetaminophen via Optical Trapping2024

    • Author(s)
      Wen-Chi Wang,Teruki Sugiyama,Kazuki Okano,Hiroshi Yoshikawa
    • Organizer
      2024 Chemistry National Meeting(CNM2024)
  • [Presentation] Advanced laser processing and manipulation techniques for life/material sciences.2024

    • Author(s)
      Hiroshi Yoshikawa
    • Organizer
      Kyoto Winter School 2024 “Towards Holistic Understanding of Life”
    • Invited
  • [Presentation] 集光レーザービームによるコロイド結晶形成制御法の探索2023

    • Author(s)
      藤井紀文,高橋秀実,松﨑賢寿,吉川洋史
    • Organizer
      レーザー学会 関西支部中国・四国支部連合
  • [Presentation] Control of self-organization of organoids by mechanically patterned hydrogel2023

    • Author(s)
      Takahisa Matsuzaki, Hiroshi Yoshikawa
    • Organizer
      9th International Conference on Mechanics of Biomaterials and Tissues
    • Int'l Joint Research
  • [Presentation] Photophysical approach for fabrication of dynamic biomolecular assembly2023

    • Author(s)
      Hiroshi Yoshikawa,Shuma Matsumoto,Ren Shirata,Takahisa Matsuzaki,Genki Fukasawa,Tomoaki Matsuura,Teruki Sugiyama,Ryuzo Kawamura
    • Organizer
      9th International Conference on Mechanics of Biomaterials and Tissues
    • Int'l Joint Research
  • [Presentation] Chemically crosslinked microtubules driven by kinesins as an active substrate for cells2023

    • Author(s)
      Ryuzo Kawamura, Kaito Motoyoshi, Tsuyoshi Yokoyama, Takahisa Matsuzaki, Hiroshi Y Yoshikawa, Tomonori Kawasaki
    • Organizer
      MRM2023/IUMRS-ICA2023
    • Int'l Joint Research
  • [Presentation] フラーレン結晶の成長速度・形状にレーザー照射が与える影響の評価2023

    • Author(s)
      伏本航,高橋秀実,丸山美帆子,鈴木凌,橘勝,吉川洋史
    • Organizer
      第52回結晶成長国内会議JCCG-52
  • [Presentation] Spatiotemporal control of condensation of biomolecules via photophysical effects of a focused laser beam2023

    • Author(s)
      Shuma Matsumoto,Ren Shirata,Genki Fukasawa,Takahisa Matsuzaki,Teruki Sugiyama Ryuzo Kawamura,Tomoaki Matsuura,Hiroshi Yoshikawa
    • Organizer
      第61回日本生物物理学会年会
  • [Presentation] Influence of pulsed laser irradiation on dissolution behavior2023

    • Author(s)
      Anna Konishi,Erika Yamashita, Mihoko Maruyama,Takahisa Matsuzaki,Heqi Xi,Menglu Li,Katsumasa Fujita,Junichi Kikuta,Yusuke Mori,Masaru Ishii,Hiroshi Yoshikawa
    • Organizer
      第61回日本生物物理学会年会
  • [Presentation] 有機溶媒系におけるレーザー誘起結晶化ダイナミクスの解明2023

    • Author(s)
      清水 大督,高橋 秀実,釣優 香,丸山 美帆子,吉村 政志,森 勇介,吉川 洋史
    • Organizer
      2023年度第2回講演会応用物理学会関西支部
  • [Presentation] 超短パルスレーザーによる酢酸ナトリウムの擬似多形誘起とそのダイナミクス2023

    • Author(s)
      高岡 佑介,高橋 秀実,海老原 里美,釣 優香,丸山 美帆子,吉村 政志,森 勇介,吉川 洋史
    • Organizer
      2023年度第2回講演会応用物理学会関西支部
  • [Presentation] 集光レーザービームがフラーレン結晶成長に与える影響の評価2023

    • Author(s)
      伏本航,高橋秀実,丸山美帆子,鈴木凌, 橘勝,吉川洋史
    • Organizer
      2023年大阪大学JSAP学生チャプター講演会
  • [Presentation] レーザーによる機能性有機材料のボトムアップ構築2023

    • Author(s)
      伏本航,高橋秀実,吉川洋史
    • Organizer
      学術変革領域研究A 第2回若手会
  • [Presentation] Crystal nucleation, growth, and phase transition induced by focused irradiation with a pulsed laser2023

    • Author(s)
      Hiroshi Yoshikawa
    • Organizer
      International Workshop on Laser Material Processing and Applications(LMPA 2023)
    • Int'l Joint Research / Invited
  • [Presentation] フェムト秒レーザーを用いた尿路結石破砕法の検討~新規内視鏡的治療法の開拓に向けて~2023

    • Author(s)
      吉村侑大,濵本周造,丸山美帆子,田中勇太朗,高野和文,吉村正志,宇佐美茂佳,今西正幸,田尻理恵,岡田淳志,安井孝周,森勇介,吉川洋史
    • Organizer
      日本尿路結石症学会第33回学術集会
  • [Presentation] Growth Promotion of Targeted Crystal Faces of Organics Compounds and Proteins by Nano-Processing via Laser Ablation2023

    • Author(s)
      Hiroshi Y. Yoshikwa,Yuka Tsuri,Hozumi Takahashi,Megumi Shiraiwa,Yusuke Takaoka,Satomi Ebihara,Katsuo Tsukamoto,Takashi Onuma,Ryutaro Shimada,Tomohiko Tateshima,Kazufumi Takano,Hiroaki Adachi,Masashi Yoshimura,Yoichiroh Hosokawa, Seiichiro Nakabayashi,Mihoko Maruyama,Yusuke Mori
    • Organizer
      The International Conference on Crystal Growth and Epitaxy ICCGE-20
    • Int'l Joint Research
  • [Presentation] Spatiotemporal Control of Ice Crystallization in Supercooled Water by Focused Irradiation with Laser Pulses2023

    • Author(s)
      Hozumi Takahashi,Tatsuya Kono,Kosuke Sawada,Satoru Kumano,Yuka Tsuri,Mihoko Maruyama,Masashi Yoshimura,Daisuke Takahashi, Yukio Kawamura,Matsuo Uemura,Seiichiro Nakabayashi,Yusuke Mori, Yoichiroh Hosokawa, Hiroshi Y. Yoshikawa.
    • Organizer
      The International Conference on Crystal Growth and Epitaxy ICCGE-20
    • Int'l Joint Research
  • [Presentation] Optical trapping-controlled co-crystallization in two-component solution2023

    • Author(s)
      Wen-Chi Wang,Kazuki Okano,Hiroshi Y. Yoshikawa,and Teruki Sugiyama
    • Organizer
      31st International Conference on Photochemistry(ICP2023)
    • Int'l Joint Research
  • [Presentation] Spatial Control of Polymorphic Phase Transition from β-Form of Glycine Crystals by Focused Irradiation with a Femtosecond Laser Pulse2023

    • Author(s)
      Hozumi Takahashi,Yudai Yoshimura,Ryota Murai,Ryuzo Kawamura,Mihoko Maruyama,Masashi Yoshimura,Yusuke Mori,Hiroshi Y Yoshikawa
    • Organizer
      31st International Conference on Photochemistry(ICP2023)
    • Int'l Joint Research
  • [Presentation] High-speed observation of ice crystallization dynamics in supercooled water triggered by laser ablation2023

    • Author(s)
      Hozumi Takahashi, Tatsuya Kono, Kosuke Sawada, Satoru Kumano, Yuka Tsuri, Mihoko Maruyama, Masashi Yoshimura, Daisuke Takahashi, Yukio Kawamura, Matsuo Uemura, Seiichiro Nakabayashi, Yusuke Mori, Yoichiroh Hosokawa, and Hiroshi Y. Yoshikawa
    • Organizer
      The 24th International Symposium on Laser Precision Microfabrication
    • Int'l Joint Research
  • [Presentation] High-speed imaging of laser ablation in supersaturated solutions towards understanding of laser-induced crystallization mechanism2023

    • Author(s)
      Hiroshi Yoshikawa,Yuka Tsuri,Hozumi Takahasahi,Megumi Shiraiwa,Katsuo Tsukamoto,Takashi Onuma,Ryutaro Shimada,Tomohiko Tateshima,Kazufumi Takano,Hiroaki Adachi,Masahi Yoshimura,Yoichiroh Hosokawa,Seiichiro Nakabayashi,Mihoko Maruyama,Yusuke Mori
    • Organizer
      The 24th International Symposium on Laser Precision Microfabrication
    • Int'l Joint Research
  • [Presentation] Femtosecond laser processing of kidney stones2023

    • Author(s)
      Yudai Yoshimura,Shuzo Hamamoto,Mihoko Maruyama,Masashi Yoshimura,Shigeyuki Usami,Masayuki Imanishi, Rie Tajiri,Yutaro Tanaka,Atsushi Okada,Kazufumi Takano,Takahiro Yasui,Yusuke Mori,Hiroshi Y Yoshikawa
    • Organizer
      The 24th International Symposium on Laser Precision Microfabrication
    • Int'l Joint Research
  • [Presentation] Production of Organic Functional Crystals by Using Intensive Lasers2023

    • Author(s)
      高岡佑介,海老原里美,高橋秀実,釣優香,丸山美帆子,吉村政志,森勇介,吉川洋史
    • Organizer
      光・量子ビーム科学合同シンポジウム(OPTO2023)
  • [Presentation] レーザーアブレーションによる有機非線形光学結晶の液中加工2023

    • Author(s)
      高橋秀実,白岩愛実,山地真由,Valynn K. Mag-usara,北原英明,中嶋誠,丸山美帆子,吉村政志,森勇介,杉山輝樹,中林誠一郎,吉川洋史
    • Organizer
      応用物理学会関西支部2023年度第1回講演会

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Published: 2024-12-25  

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