Development of high-throughput human protein expression and purification system in silkworms
Project/Area Number |
23760747
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Biofunction/Bioprocess
|
Research Institution | Shizuoka University |
Principal Investigator |
TATSUYA Kato 静岡大学, (連合)農学研究科(研究院), 准教授 (00397366)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | カイコ / ハイスループット / タンパク質発現 / かいこさなぎ / FLAGタグ / タンパク質精製 |
Research Abstract |
Multi-expression and purification of recombinant proteins in BmNPV bacmid-silkworm expression system were constructed. Many kinds of recombinant BmNPV bacmid DNA were prepared in a 96 well plate and recombinant proteins were also purified in a 96 well plate packing a purification gel. The further improvement of protein extraction and purification step leads to the establishment of a more practical silkworm system.
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Report
(4 results)
Research Products
(7 results)